Application
Western blot analysis of TOLLIP (arrow) using rabbit polyclonal TOLLIP Antibody (G145) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TOLLIP gene.Application
TOLLIP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TOLLIP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TOLLIP Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TOLLIP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOLLIP; Toll-interacting protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TOLLIP Toll-interacting protein |
|---|---|
| Molecular Weight(MW) | 30282 Da |
| Function | Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity |
| Tissue Specificity | This TOLLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138~167 amino acids from the center region of human TOLLIP. |
| Cellular Localization | Cytoplasm (Probable). |
Application
Western blot analysis of TOLLIP (arrow) using rabbit polyclonal TOLLIP Antibody (G145) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TOLLIP gene.
Application
TOLLIP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TOLLIP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TOLLIP Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
