Application
TOP3B Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TOP3B antibody detected the TOP3B protein (arrow).| Product Name | TOP3B Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOP3B; TOP3B1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TOP3B1 |
|---|---|
| Alternative Names | TOP3B TOP3B1 DNA topoisomerase 3-beta-1 DNA topoisomerase III beta-1 |
| Molecular Weight(MW) | 96662 Da |
| Function | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity |
| Tissue Specificity | Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas |
| Entrez Gene | 8940 |
|---|---|
| Nucleotide Accession | NP_003926.1 |
Application
TOP3B Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TOP3B antibody detected the TOP3B protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
