Application
TOPBP1 Antibody (S1138) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TOPBP1 antibody detected the TOPBP1 protein (arrow).| Product Name | TOPBP1 Antibody (Center S1138) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOPBP1; KIAA0259; DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-beta-binding protein 1; DNA topoisomerase II-binding protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0259 |
|---|---|
| Alternative Names | TOPBP1 KIAA0259 DNA topoisomerase 2-binding protein 1 DNA topoisomerase II-beta-binding protein 1 DNA topoisomerase II-binding protein 1 |
| Molecular Weight(MW) | 170679 Da |
| Function | Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double- stranded DNA breaks and nicks as well as single-stranded DNA Recruits the SWI/SNF chromatin remodeling complex to E2F1- responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR |
| Tissue Specificity | Highly expressed in heart, brain, placenta, lung and kidney |
| Cellular Localization | Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome Note=Detected on unpaired autosomes in meiotic prophase cells Detected on X and Y chromosomes during later stages of prophase Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner |
| Entrez Gene | 11073 |
|---|---|
| Nucleotide Accession | NP_008958.2 |
Application
TOPBP1 Antibody (S1138) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TOPBP1 antibody detected the TOPBP1 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
