Application
Western blot analysis of TP73 Antibody (Center) in 293 cell line lysates (35ug/lane). TP73 (arrow) was detected using the purified Pab.Application
Detects in HCT116 cell line lysates with TP73 Antibody (Center) which comes from Abgent(20ug/lane). (Kindly supplied by Doc. Wu)Application
TP73 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TP73 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TP73 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of TP73 Antibody (Center) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | TP73 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TP73; P73; Tumor protein p73; p53-like transcription factor; p53-related protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | P73 |
|---|---|
| Alternative Names | TP73 P73 Tumor protein p73 p53-like transcription factor p53-related protein |
| Molecular Weight(MW) | 69623 Da |
| Function | Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein |
| Tissue Specificity | Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue |
| Cellular Localization | Nucleus. Cytoplasm. Note=Accumulates in the nucleus in response to DNA damage |
| Entrez Gene | 7161 |
|---|
Application
Western blot analysis of TP73 Antibody (Center) in 293 cell line lysates (35ug/lane). TP73 (arrow) was detected using the purified Pab.
Application
Detects in HCT116 cell line lysates with TP73 Antibody (Center) which comes from Abgent(20ug/lane). (Kindly supplied by Doc. Wu)
Application
TP73 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TP73 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TP73 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of TP73 Antibody (Center) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
