Application
TRAF6 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TRAF6 antibody detected the TRAF6 protein (arrow).| Product Name | TRAF6 (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRAF6; RNF85; TNF receptor-associated factor 6; E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer; RING finger protein 85 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF85 |
|---|---|
| Alternative Names | TRAF6 RNF85 TNF receptor-associated factor 6 E3 ubiquitin-protein ligase TRAF6 Interleukin-1 signal transducer RING finger protein 85 |
| Molecular Weight(MW) | 59573 Da |
| Function | E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF- kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL- 1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation |
| Tissue Specificity | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas |
| Cellular Localization | Cytoplasm. Cytoplasm, cell cortex. Nucleus. Lipid droplet (By similarity). Note=Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T- and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus. RSAD2/viperin recruits it to the lipid droplet (By similarity) |
| Entrez Gene | 7189 |
|---|---|
| Nucleotide Accession | NP_665802.1 |
Application
TRAF6 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TRAF6 antibody detected the TRAF6 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
