Application
A. Western blot analysis on Jurkat cell lysate using anti-TRAF6 (C-term) RabMAb, dilution 1:1000.Application
B. Immunofluorescent staining of HeLa cells using anti-TRAF-6 RabMAb| Product Name | TRAF6 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRAF6, RNF85, TNF receptor-associated factor 6, Interleukin-1 signal transducer;RING finger protein 85 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | Cocktail |
| Clone Number | EP592Y |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| WB:1:1000 IF:1:100~1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RNF85 |
|---|---|
| Alternative Names | TRAF6 RNF85 TNF receptor-associated factor 6 Interleukin-1 signal transducer RING finger protein 85 |
| Molecular Weight(MW) | 59573 Da |
| Function | E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF- kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL- 1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation |
| Tissue Specificity | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas |
| Cellular Localization | Cytoplasm. Cytoplasm, cell cortex. Nucleus. Lipid droplet (By similarity). Note=Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T- and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus. RSAD2/viperin recruits it to the lipid droplet (By similarity) |
| Entrez Gene | 7189 |
|---|
Application
A. Western blot analysis on Jurkat cell lysate using anti-TRAF6 (C-term) RabMAb, dilution 1:1000.
Application
B. Immunofluorescent staining of HeLa cells using anti-TRAF-6 RabMAb| Application Notes | WB:1:1000 IF:1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
