Application
Western blot analysis of TRAP antibody (N-term) (Cat.# AP6545a) in NCI-H460 cell line lysates (35ug/lane). TRAP (arrow) was detected using the purified Pab.Application
TRAP Antibody (N-term) (Cat.# AP6545a) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of TRAP Antibody (N-term)(Cat#AP6545a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
NQO1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TRAP Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD40LG; CD40L; TNFSF5; TRAP; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; Tumor necrosis factor ligand superfamily member 5; CD_antigen=CD154; CD40 ligand, membrane form; CD40 ligand, soluble form |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD40L TNFSF5 TRAP |
|---|---|
| Alternative Names | CD40LG CD40L TNFSF5 TRAP CD40 ligand T-cell antigen Gp39 TNF-related activation protein Tumor necrosis factor ligand superfamily member 5 CD_antigen=CD154 CD40 ligand membrane form CD40 ligand soluble form |
| Molecular Weight(MW) | 29274 Da |
| Function | Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4 Involved in immunoglobulin class switching |
| Tissue Specificity | Specifically expressed on activated CD4+ T- lymphocytes |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein |
| Entrez Gene | 959 |
|---|
Application
Western blot analysis of TRAP antibody (N-term) (Cat.# AP6545a) in NCI-H460 cell line lysates (35ug/lane). TRAP (arrow) was detected using the purified Pab.
Application
TRAP Antibody (N-term) (Cat.# AP6545a) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRAP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of TRAP Antibody (N-term)(Cat#AP6545a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
NQO1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
