Application
Western blot analysis of CD40L expression with lysates from Jurkat cells (Lane 1), mouse small intestine (Lane 2) and rat kidney (Lane 3).| Product Name | CD40L / CD154 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CD40LCD40 ligand (CD40L), also known as CD154, gp39 and TRAP, is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X-linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes. CD40, also known as bp50 or CDW40, is a type I membrane protein found on the surface of B cells and primary carcinomas. It belongs to the TNF-R family. CD40 serves as the receptor for CD40 ligand (CD40L), a cytokine expressed on the cell surface of T cells. CD40 plays a critical role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with T cells expressing CD40L, and activation of CD4+ T cells. Ligation of CD40 with its ligand preferentially induces stress-activated protein kinases in B lymphocytes. |
| Immunogen | Highly purified recombinant human CD40L (CD40 Ligand) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB: Use at a concentration of 0.5 - 1 μg/ml. ELISA: Use at a concentration of 2-3 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TRAP IMD3 CD40L 300386 959 IGM TNFSF5 HIGM1 CD40LG CD154 gp39 TBAM hCD40L P29965 T-BAM T BAM |
|---|---|
| Target | CD40L |
Application
Western blot analysis of CD40L expression with lysates from Jurkat cells (Lane 1), mouse small intestine (Lane 2) and rat kidney (Lane 3).| Application Notes | WB: Use at a concentration of 0.5 - 1 μg/ml. ELISA: Use at a concentration of 2-3 μg/ml. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 1 mg/ml BSA, 1.5 mM sodium azide and 50% glycerol |
