Application
A. Western blot analysis on (A) Daudi and (B) U937 cell lysates using anti-Transcription factor PU.1 RabMAb (Cat. #AJ1789b), dilution 1:10,000.Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Transcription factor PU.1 RabMAb (Cat. #AJ1789b).| Product Name | Transcription factor PU.1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPI1, Transcription factor PU.1, 31 kDa-transforming protein |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3158Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:5000~20000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | SPI1 Transcription factor PU.1 31 kDa-transforming protein |
|---|---|
| Molecular Weight(MW) | 31083 Da |
| Function | Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity) |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N terminus of Transcription factor PU.1 was used as an immunogen. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 6688 |
|---|
Application
A. Western blot analysis on (A) Daudi and (B) U937 cell lysates using anti-Transcription factor PU.1 RabMAb (Cat. #AJ1789b), dilution 1:10,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Transcription factor PU.1 RabMAb (Cat. #AJ1789b).| Application Notes | WB:1:5000~20000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
