Application
TRIM24 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the TRIM24 antibody detected the TRIM24 protein (arrow).| Product Name | TRIM24 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM24; RNF82; TIF1; TIF1A; Transcription intermediary factor 1-alpha; E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; Tripartite motif-containing protein 24 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF82 TIF1 TIF1A |
|---|---|
| Alternative Names | TRIM24 RNF82 TIF1 TIF1A Transcription intermediary factor 1-alpha E3 ubiquitin-protein ligase TRIM24 RING finger protein 82 Tripartite motif-containing protein 24 |
| Molecular Weight(MW) | 116831 Da |
| Function | Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity) |
| Tissue Specificity | This TRIM24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human TRIM24. |
| Cellular Localization | Nucleus. Cytoplasm. Note=Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear |
| Entrez Gene | 8805 |
|---|---|
| Nucleotide Accession | NP_003843.3 |
Application
TRIM24 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the TRIM24 antibody detected the TRIM24 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
