Application
TRIM33 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the TRIM33 antibody detected the TRIM33 protein (arrow).| Product Name | TRIM33 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM33; KIAA1113; RFG7; TIF1G; E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; RET-fused gene 7 protein; Transcription intermediary factor 1-gamma; Tripartite motif-containing protein 33 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1113 RFG7 TIF1G |
|---|---|
| Alternative Names | TRIM33 KIAA1113 RFG7 TIF1G E3 ubiquitin-protein ligase TRIM33 Ectodermin homolog RET-fused gene 7 protein Transcription intermediary factor 1-gamma Tripartite motif-containing protein 33 |
| Molecular Weight(MW) | 122533 Da |
| Function | Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade) |
| Tissue Specificity | Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors |
| Cellular Localization | Nucleus. Note=In discrete nuclear dots resembling nuclear bodies (By similarity) |
| Entrez Gene | 51592 |
|---|---|
| Nucleotide Accession | NP_148980.2 NP_056990.3 |
Application
TRIM33 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the TRIM33 antibody detected the TRIM33 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
