Application
TRIM65 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TRIM65 antibody detected the TRIM65 protein (arrow).Application
TRIM65 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM65 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of TRIM65 Antibody (Center)(Cat#AP11814c) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
TRIM65 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TRIM65 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIM65; Tripartite motif-containing protein 65 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TRIM65 Tripartite motif-containing protein 65 |
|---|---|
| Molecular Weight(MW) | 57353 Da |
| Tissue Specificity | This TRIM65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 334-364 amino acids from the Central region of human TRIM65. |
| Entrez Gene | 201292 |
|---|---|
| Nucleotide Accession | NP_775818.2 |
Application
TRIM65 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TRIM65 antibody detected the TRIM65 protein (arrow).
Application
TRIM65 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM65 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of TRIM65 Antibody (Center)(Cat#AP11814c) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
TRIM65 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
