Application
Western blot analysis of TRIP13 (arrow) using rabbit polyclonal TRIP13 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TRIP13 gene.Application
TRIP13 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIP13 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TRIP13 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TRIP13 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRIP13; PCH2; Pachytene checkpoint protein 2 homolog; Human papillomavirus type 16 E1 protein-binding protein; Thyroid hormone receptor interactor 13; Thyroid receptor-interacting protein 13 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PCH2 |
|---|---|
| Alternative Names | TRIP13 PCH2 Pachytene checkpoint protein 2 homolog Human papillomavirus type 16 E1 protein-binding protein Thyroid hormone receptor interactor 13 Thyroid receptor-interacting protein 13 |
| Molecular Weight(MW) | 48551 Da |
| Function | Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double- strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes (By similarity) |
| Tissue Specificity | This TRIP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 370-400 amino acids from the C-terminal region of human TRIP13. |
| Entrez Gene | 9319 |
|---|---|
| Nucleotide Accession | NP_004228.1 |
Application
Western blot analysis of TRIP13 (arrow) using rabbit polyclonal TRIP13 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TRIP13 gene.
Application
TRIP13 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIP13 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TRIP13 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
