Application
Western blot analysis of TRMT5 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). TRMT5 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with TRMT5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
TRMT5 Antibody (N-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TRMT5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRMT5; KIAA1393; TRM5; tRNA (guanine(37)-N1)-methyltransferase; M1G-methyltransferase; tRNA [GM37] methyltransferase; tRNA methyltransferase 5 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1393 TRM5 |
|---|---|
| Alternative Names | TRMT5 KIAA1393 TRM5 tRNA (guanine(37)-N1)-methyltransferase M1G-methyltransferase tRNA [GM37] methyltransferase tRNA methyltransferase 5 homolog |
| Molecular Weight(MW) | 58246 Da |
| Function | Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding |
| Tissue Specificity | This TRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115~145 amino acids from the N-terminal region of human TRMT5. |
| Cellular Localization | Mitochondrion matrix (By similarity). Nucleus (By similarity). Cytoplasm (By similarity) Note=Predominantly in the mitochondria and in the nucleus (By similarity) |
| Entrez Gene | 57570 |
|---|
Application
Western blot analysis of TRMT5 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). TRMT5 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with TRMT5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
TRMT5 Antibody (N-term) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
