Application
"Western blot analysis of TRPM8 (arrow) using rabbit polyclonal TRPM8 Antibody (Center R536).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRPM8 gene (Lane 2) (Origene Technologies)."Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TRPM8 antibody (Center R536) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | TRPM8 Antibody (Center R536) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRPM8; LTRPC6; TRPP8; Transient receptor potential cation channel subfamily M member 8; Long transient receptor potential channel 6; Transient receptor potential p8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | LTRPC6 TRPP8 |
|---|---|
| Alternative Names | TRPM8 LTRPC6 TRPP8 Transient receptor potential cation channel subfamily M member 8 Long transient receptor potential channel 6 Transient receptor potential p8 |
| Molecular Weight(MW) | 127685 Da |
| Function | Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium Temperature sensing is tightly linked to voltage-dependent gating Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity |
| Tissue Specificity | Expressed in prostate. Also expressed in prostate tumors and in non-prostatic primary tumors such as colon, lung, breast and skin tumors |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Membrane raft. Endoplasmic reticulum membrane. Note=Localizes to membrane rafts but is also located in the cell membrane outside of these regions where channel response to cold is enhanced compared to membrane rafts (By similarity). Located in the endoplasmic reticulum in prostate cancer cells |
| Entrez Gene | 79054 |
|---|
Application
"Western blot analysis of TRPM8 (arrow) using rabbit polyclonal TRPM8 Antibody (Center R536).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRPM8 gene (Lane 2) (Origene Technologies)."
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TRPM8 antibody (Center R536) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
