Application
Western blot analysis of anti-CHAK1 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). CHAK1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
CHAK1 Antibody (F1835) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHAK1 antibody detected the CHAK1 protein (arrow).| Product Name | TRPM7 (CHAK1) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRPM7; CHAK1; LTRPC7; Transient receptor potential cation channel subfamily M member 7; Channel-kinase 1; Long transient receptor potential channel 7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~200 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CHAK1 LTRPC7 |
|---|---|
| Alternative Names | TRPM7 CHAK1 LTRPC7 Transient receptor potential cation channel subfamily M member 7 Channel-kinase 1 Long transient receptor potential channel 7 |
| Molecular Weight(MW) | 212697 Da |
| Function | Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell Phosphorylates annexin A1 (ANXA1) |
| Tissue Specificity | This TRPM7 (CHAK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1827~1857 amino acids from the C-terminal region of human CHAK1. |
| Cellular Localization | Membrane; Multi-pass membrane protein (Probable) |
Application
Western blot analysis of anti-CHAK1 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). CHAK1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
CHAK1 Antibody (F1835) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHAK1 antibody detected the CHAK1 protein (arrow).| Application Notes | WB:1:50~200 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Data sheet for OM223715