Application
TSC22D2 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TSC22D2 antibody detected the TSC22D2 protein (arrow).Application
Fluorescent confocal image of A431 cell stained with TSC22D2 Antibody (N-term)(Cat#AP16530a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC22D2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TSC22D2 immunoreactivity is localized to Cytoplasm significantly.| Product Name | TSC22D2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSC22D2; KIAA0669; TILZ4; TSC22 domain family protein 2; TSC22-related-inducible leucine zipper protein 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0669 TILZ4 |
|---|---|
| Alternative Names | TSC22D2 KIAA0669 TILZ4 TSC22 domain family protein 2 TSC22-related-inducible leucine zipper protein 4 |
| Molecular Weight(MW) | 79228 Da |
| Tissue Specificity | This TSC22D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TSC22D2. |
| Entrez Gene | 9819 |
|---|---|
| Nucleotide Accession | NP_055594.1 |
Application
TSC22D2 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TSC22D2 antibody detected the TSC22D2 protein (arrow).
Application
Fluorescent confocal image of A431 cell stained with TSC22D2 Antibody (N-term)(Cat#AP16530a).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC22D2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TSC22D2 immunoreactivity is localized to Cytoplasm significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
