Application
TSEN2 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TSEN2 antibody detected the TSEN2 protein (arrow).| Product Name | TSEN2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSEN2; SEN2; tRNA-splicing endonuclease subunit Sen2; tRNA-intron endonuclease Sen2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SEN2 |
|---|---|
| Alternative Names | TSEN2 SEN2 tRNA-splicing endonuclease subunit Sen2 tRNA-intron endonuclease Sen2 |
| Molecular Weight(MW) | 53247 Da |
| Function | Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre- mRNA 3'-end processing factors, establishing a link between pre- tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity |
| Tissue Specificity | Isoform 1 and isoform 2 are widely expressed at very low level |
| Cellular Localization | Nucleus. Nucleus, nucleolus. Note=May be transiently localized in the nucleolus |
| Entrez Gene | 80746 |
|---|---|
| Nucleotide Accession | NP_001138864.1 |
Application
TSEN2 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TSEN2 antibody detected the TSEN2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
