Application
Western blot analysis of TSH2 Antibody (N-term) in CEM cell line lysates (35ug/lane).TSH2 (arrow) was detected using the purified Pab.Application
TSH2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TSH2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TSH2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Fluorescent confocal image of U251 cell stained with TSH2 Antibody (N-term)(Cat#AP5288a).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSH2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TSH2 immunoreactivity is localized to Nucleus and Vesicles significantly.| Product Name | TSH2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSHZ2; C20orf17; TSH2; ZNF218; Teashirt homolog 2; Ovarian cancer-related protein 10-2; Zinc finger protein 218 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C20orf17 TSH2 ZNF218 |
|---|---|
| Alternative Names | TSHZ2 C20orf17 TSH2 ZNF218 Teashirt homolog 2 Ovarian cancer-related protein 10-2 Zinc finger protein 218 |
| Molecular Weight(MW) | 115005 Da |
| Function | Probable transcriptional regulator involved in developmental processes. May act as a transcriptional repressor (Potential) |
| Tissue Specificity | Expressed in brain; strongly reduced in post- mortem elderly subjects with Alzheimer disease |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 128553 |
|---|
Application
Western blot analysis of TSH2 Antibody (N-term) in CEM cell line lysates (35ug/lane).TSH2 (arrow) was detected using the purified Pab.
Application
TSH2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TSH2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TSH2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Fluorescent confocal image of U251 cell stained with TSH2 Antibody (N-term)(Cat#AP5288a).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSH2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TSH2 immunoreactivity is localized to Nucleus and Vesicles significantly.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
