Application
TTBK2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TTBK2 antibody detected the TTBK2 protein (arrow).Application
TTBK2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TTBK2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
TTBK2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TTBK2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TTBK2; KIAA0847; Tau-tubulin kinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0847 |
|---|---|
| Alternative Names | TTBK2 KIAA0847 Tau-tubulin kinase 2 |
| Molecular Weight(MW) | 137412 Da |
| Function | Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro |
| Tissue Specificity | This TTBK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-253 amino acids from the N-terminal region of human TTBK2. |
| Cellular Localization | Cell projection, cilium (By similarity). Cytoplasm, cytoskeleton, cilium basal body (By similarity) Cytoplasm, cytoskeleton, centrosome, centriole. Cytoplasm, cytosol. Nucleus. Note=Localizes to the transition zone in primary cilia in response to cell cycle signals that promote ciliogenesis (By similarity). May also be present in cytosol and, at lower level in the nucleus |
| Entrez Gene | 146057 |
|---|---|
| Nucleotide Accession | NP_775771.3 |
Application
TTBK2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TTBK2 antibody detected the TTBK2 protein (arrow).
Application
TTBK2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TTBK2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
TTBK2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
