Application
Western blot analysis of TTF2 Antibody (N-term) in HL60 cell line lysates (35ug/lane). TTF2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with TTF2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
TTF2 Antibody (N-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TTF2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TTF2; Transcription termination factor 2; Lodestar homolog; RNA polymerase II termination factor; Transcription release factor 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TTF2 Transcription termination factor 2 Lodestar homolog RNA polymerase II termination factor Transcription release factor 2 |
|---|---|
| Molecular Weight(MW) | 129588 Da |
| Function | DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing |
| Tissue Specificity | This TTF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57~87 amino acids from the N-terminal region of human TTF2. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic during interphase. Relocates to the nucleus as cells enter mitosis |
| Entrez Gene | 8458 |
|---|
Application
Western blot analysis of TTF2 Antibody (N-term) in HL60 cell line lysates (35ug/lane). TTF2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with TTF2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
TTF2 Antibody (N-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
