Application
Confocal immunofluorescent analysis of TUBB2B Antibody (N-term)(Cat#AP11940a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
TUBB2B Antibody (N-term) western blot analysis in HepG2,Hela,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TUBB2B antibody detected the TUBB2B protein (arrow).Application
TUBB2B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TUBB2B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | TUBB2B Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TUBB2B; Tubulin beta-2B chain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBIHC |
| IF:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TUBB2B Tubulin beta-2B chain |
|---|---|
| Molecular Weight(MW) | 49953 Da |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). TUBB2B is implicated in neuronal migration |
| Tissue Specificity | High expression in brain. |
| Cellular Localization | Cytoplasm, cytoskeleton (By similarity). |
| Entrez Gene | 347733 |
|---|---|
| Nucleotide Accession | NP_821080.1 |
Application
Confocal immunofluorescent analysis of TUBB2B Antibody (N-term)(Cat#AP11940a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
TUBB2B Antibody (N-term) western blot analysis in HepG2,Hela,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TUBB2B antibody detected the TUBB2B protein (arrow).
Application
TUBB2B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TUBB2B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | IF:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
