Application
Western blot analysis of TSC2-pS1798 in Ramos cell line lysates (35ug/lane). TSC2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TSC2 Antibody (S1798) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Tuberin (TSC2) Antibody (S1798) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TSC2; TSC4; Tuberin; Tuberous sclerosis 2 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TSC4 |
|---|---|
| Alternative Names | TSC2 TSC4 Tuberin Tuberous sclerosis 2 protein |
| Molecular Weight(MW) | 200608 Da |
| Function | In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase- activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors |
| Tissue Specificity | Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein. Note=At steady state found in association with membranes |
| Entrez Gene | 7249 |
|---|
Application
Western blot analysis of TSC2-pS1798 in Ramos cell line lysates (35ug/lane). TSC2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with TSC2 Antibody (S1798) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM223918