Application
TUT1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TUT1 antibody detected the TUT1 protein (arrow).Application
TUT1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TUT1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TUT1; RBM21; Speckle targeted PIP5K1A-regulated poly(A) polymerase; RNA-binding motif protein 21; U6 snRNA-specific terminal uridylyltransferase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RBM21 |
|---|---|
| Alternative Names | TUT1 RBM21 Speckle targeted PIP5K1A-regulated poly(A) polymerase RNA-binding motif protein 21 U6 snRNA-specific terminal uridylyltransferase 1 |
| Molecular Weight(MW) | 93847 Da |
| Function | Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication- dependent histone mRNA degradation |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Nucleus, nucleolus. Nucleus speckle. |
| Entrez Gene | 64852 |
|---|---|
| Nucleotide Accession | NP_073741.1 |
Application
TUT1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TUT1 antibody detected the TUT1 protein (arrow).
Application
TUT1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
