Application
Western blot analysis of Tyrosinase Antibody (Center) in A375, 293 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of TYR Antibody (Center)(Cat#AP7345c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
TYR Antibody (Center) (AP7345c)immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | TYR Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TYR; Tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TYR Tyrosinase LB24-AB Monophenol monooxygenase SK29-AB Tumor rejection antigen AB |
|---|---|
| Molecular Weight(MW) | 60393 Da |
| Function | This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone |
| Tissue Specificity | This TYR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183~213 amino acids from the Center region of human Tyrosinase. |
| Cellular Localization | Melanosome membrane; Single-pass type I membrane protein |
| Entrez Gene | 7299 |
|---|
Application
Western blot analysis of Tyrosinase Antibody (Center) in A375, 293 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of TYR Antibody (Center)(Cat#AP7345c) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
TYR Antibody (Center) (AP7345c)immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
