Application
Western blot analysis of TYRO10 (arrow) using rabbit polyclonal TYRO10 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDR2 gene (Lane 2) (Origene Technologies).Application
Western blot analysis of TYRO10 Antibody in Jurkat(lane 1), 293(lane 2) and Hela(lane 3) cell line lysates (35ug/lane). TYRO10 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human normal lung with TYRO10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of jurkat cells using TYRO10 Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TYRO10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DDR2; NTRKR3; TKT; TYRO10; Discoidin domain-containing receptor 2; CD167 antigen-like family member B; Discoidin domain-containing receptor tyrosine kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; Tyrosine |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NTRKR3 TKT TYRO10 |
|---|---|
| Alternative Names | DDR2 NTRKR3 TKT TYRO10 Discoidin domain-containing receptor 2 CD167 antigen-like family member B Discoidin domain-containing receptor tyrosine kinase 2 Neurotrophic tyrosine kinase receptor-related 3 Receptor protein-tyrosine kinase TKT Tyrosine |
| Molecular Weight(MW) | 96736 Da |
| Function | Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing |
| Tissue Specificity | Detected in osteocytes, osteoblastic cells in subchondral bone, bone lining cells, tibia and cartilage (at protein level). Detected at high levels in heart and lung, and at low levels in brain, placenta, liver, skeletal muscle, pancreas, and kidney |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 4921 |
|---|
Application
Western blot analysis of TYRO10 (arrow) using rabbit polyclonal TYRO10 Antibody . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDR2 gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of TYRO10 Antibody in Jurkat(lane 1), 293(lane 2) and Hela(lane 3) cell line lysates (35ug/lane). TYRO10 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human normal lung with TYRO10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of jurkat cells using TYRO10 Antibody(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
