Application
Western blot analysis of Tyrosinase antibody (C-term) in HepG2 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab.Application
Tyrosinase Antibody (C-term) (AP7345b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Tyrosinase Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of Tyrosinase Antibody (C-term)(Cat#AP7345b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Tyrosinase Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Tyrosinase Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TYR; Tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | TYR Tyrosinase LB24-AB Monophenol monooxygenase SK29-AB Tumor rejection antigen AB |
|---|---|
| Molecular Weight(MW) | 60393 Da |
| Function | This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone |
| Tissue Specificity | This Tyrosinase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 492~522 amino acids from the C-terminal region of human TYR. |
| Cellular Localization | Melanosome membrane; Single-pass type I membrane protein |
| Entrez Gene | 7299 |
|---|
Application
Western blot analysis of Tyrosinase antibody (C-term) in HepG2 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab.
Application
Tyrosinase Antibody (C-term) (AP7345b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Tyrosinase Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of Tyrosinase Antibody (C-term)(Cat#AP7345b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Tyrosinase Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
