Application
U2AF2 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the U2AF2 antibody detected the U2AF2 protein (arrow).| Product Name | U2AF2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | U2AF2; U2AF65; Splicing factor U2AF 65 kDa subunit; U2 auxiliary factor 65 kDa subunit; U2 snRNP auxiliary factor large subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | U2AF65 |
|---|---|
| Alternative Names | U2AF2 U2AF65 Splicing factor U2AF 65 kDa subunit U2 auxiliary factor 65 kDa subunit U2 snRNP auxiliary factor large subunit |
| Molecular Weight(MW) | 53501 Da |
| Function | Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10 |
| Tissue Specificity | This U2AF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human U2AF2. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 11338 |
|---|---|
| Nucleotide Accession | NP_001012496.1 NP_009210.1 |
Application
U2AF2 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the U2AF2 antibody detected the U2AF2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
