Application
U2AF4 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the U2AF4 antibody detected the U2AF4 protein (arrow).Application
U2AF4 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | U2AF4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | U2AF1L4; U2AF1-RS3; U2AF1L3; Splicing factor U2AF 26 kDa subunit; U2 auxiliary factor 26; U2 small nuclear RNA auxiliary factor 1-like protein 4; U2(RNU2) small nuclear RNA auxiliary factor 1-like protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | U2AF1-RS3 U2AF1L3 |
|---|---|
| Alternative Names | U2AF1L4 U2AF1-RS3 U2AF1L3 Splicing factor U2AF 26 kDa subunit U2 auxiliary factor 26 U2 small nuclear RNA auxiliary factor 1-like protein 4 U2(RNU2) small nuclear RNA auxiliary factor 1-like protein 3 |
| Molecular Weight(MW) | 25744 Da |
| Function | RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T-cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site (By similarity) |
| Tissue Specificity | Isoform 2 is widely expressed. Isoform 3 is highly expressed in heart, brain and lung, lower expressed in thymus and much lower expressed in peripheral blood leukocytes |
| Cellular Localization | Nucleus (By similarity). Nucleus speckle (By similarity) |
| Entrez Gene | 199746 |
|---|---|
| Nucleotide Accession | NP_659424.2 NP_001035515.1 |
Application
U2AF4 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the U2AF4 antibody detected the U2AF4 protein (arrow).
Application
U2AF4 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
