Application
Western blot analysis of TYW2 Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane). TYW2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with TYW2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
TYW2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TYW2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRMT12; TRM12; TYW2; tRNA wybutosine-synthesizing protein 2 homolog; Alpha-amino-alpha-carboxypropyl transferase TYW2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TRM12 TYW2 |
|---|---|
| Alternative Names | TRMT12 TRM12 TYW2 tRNA wybutosine-synthesizing protein 2 homolog Alpha-amino-alpha-carboxypropyl transferase TYW2 |
| Molecular Weight(MW) | 50236 Da |
| Function | Probable S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA (By similarity) |
| Tissue Specificity | This TYW2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284~313 amino acids from the C-terminal region of human TYW2. |
| Entrez Gene | 55039 |
|---|
Application
Western blot analysis of TYW2 Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane). TYW2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with TYW2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
TYW2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
