Application
UBA52 Antibody (C-Term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBA52 antibody detected the UBA52 protein (arrow).Application
UBA52 antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the UBA52 antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
UBA52 Antibody (C-Term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | UBA52 Antibody (C-Term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBA52; UBCEP2; Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1; Ubiquitin; 60S ribosomal protein L40 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UBCEP2 |
|---|---|
| Alternative Names | UBA52 UBCEP2 Ubiquitin-60S ribosomal protein L40 CEP52 Ubiquitin A-52 residue ribosomal protein fusion product 1 Ubiquitin 60S ribosomal protein L40 |
| Molecular Weight(MW) | 14728 Da |
| Function | Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling |
| Tissue Specificity | This UBA52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-136 amino acids from the C-terminal region of human UBA52. |
| Cellular Localization | Ubiquitin: Cytoplasm (By similarity). Nucleus (By similarity) |
| Entrez Gene | 7311 |
|---|---|
| Nucleotide Accession | NP_001029102.1 NP_003324.1 |
Application
UBA52 Antibody (C-Term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UBA52 antibody detected the UBA52 protein (arrow).
Application
UBA52 antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the UBA52 antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
UBA52 Antibody (C-Term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
