Application
Western blot analysis of anti-U2AF1 Antibody (Center Y114) in Ramos cell line lysates (35ug/lane).U2AF1(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with NRP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
U2AF1 Antibody (Center Y114) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of U2AF1 (arrow) using rabbit polyclonal U2AF1 Antibody (Center Y114) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.| Product Name | U2AF1 Antibody (Center Y114) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | U2AF1; U2AF35; U2AFBP; Splicing factor U2AF 35 kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | U2AF35 U2AFBP |
|---|---|
| Alternative Names | U2AF1 U2AF35 U2AFBP Splicing factor U2AF 35 kDa subunit U2 auxiliary factor 35 kDa subunit U2 small nuclear RNA auxiliary factor 1 U2 snRNP auxiliary factor small subunit |
| Molecular Weight(MW) | 27872 Da |
| Function | Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron |
| Tissue Specificity | This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105~134 amino acids from the Center region of human U2AF1. |
| Cellular Localization | Nucleus. Nucleus speckle. |
| Entrez Gene | 7307 |
|---|
Application
Western blot analysis of anti-U2AF1 Antibody (Center Y114) in Ramos cell line lysates (35ug/lane).U2AF1(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with NRP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
U2AF1 Antibody (Center Y114) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of U2AF1 (arrow) using rabbit polyclonal U2AF1 Antibody (Center Y114) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
