Application
The anti-UBE2M Pab is used in Western blot to detect UBE2M in Jurkat cell lysate.| Product Name | UBE2M Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE2M; UBC12; NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UBC12 |
|---|---|
| Alternative Names | UBE2M UBC12 NEDD8-conjugating enzyme Ubc12 NEDD8 carrier protein NEDD8 protein ligase Ubiquitin-conjugating enzyme E2 M |
| Molecular Weight(MW) | 20900 Da |
| Function | Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation |
| Tissue Specificity | This UBE2M antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154~183 amino acids from the center region of human UBE2M. |
Application
The anti-UBE2M Pab is used in Western blot to detect UBE2M in Jurkat cell lysate.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
