WB
Western blot analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBC12/UBE2M antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for UBC12/UBE2M at approximately 21 kDa. The expected band size for UBC12/UBE2M is at 21 kDa.IHC
IHC analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody. UBC12/UBE2M was detected in a paraffin-embedded section of human cervical cancer tissue. The tissue section was incubated with rabbit anti-UBC12/UBE2M Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
IF analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody. UBC12/UBE2M was detected in an immunocytochemical section of U87 cells. The section was incubated with rabbit anti-UBC12/UBE2M Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).IF-P
IF analysis using anti- UBE2M/UBC12 antibody. detected in paraffin-embedded section of human breast cancer tissue. The tissue section were stained using the Dylight®550-conjugated Anti-rabbit IgG Secondary Antibody (red)and counterstained with DAPI (blue).FC
Flow Cytometry analysis of Daudi cells using anti-UBC12/UBE2M antibody. Overlay histogram showing Daudi cells stained with anti-UBC12/UBE2M antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBC12/UBE2M Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to UBC12/UBE2M |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human UBC12/UBE2M recombinant protein (Position: K25-Q154). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBE2M |
|---|---|
| Gene Synonyms | UBC12 hUbc12 UBC-RS2 |
| Gene Full Name | ubiquitin conjugating enzyme E2 M |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008] |
| Alternative Names | hUbc12 NEDD8 carrier protein NEDD8 conjugating enzyme Ubc12 NEDD8 protein ligase UBC RS2 UBC12 UBE2M |
| Molecular Weight(MW) | 21kDa |
| Cellular Localization | cytosol,glutamatergic synapse,nucleoplasm,nucleus,postsynapse,presynapse. |
WB
Western blot analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBC12/UBE2M antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for UBC12/UBE2M at approximately 21 kDa. The expected band size for UBC12/UBE2M is at 21 kDa.
IHC
IHC analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody. UBC12/UBE2M was detected in a paraffin-embedded section of human cervical cancer tissue. The tissue section was incubated with rabbit anti-UBC12/UBE2M Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of UBC12/UBE2M using anti-UBC12/UBE2M antibody. UBC12/UBE2M was detected in an immunocytochemical section of U87 cells. The section was incubated with rabbit anti-UBC12/UBE2M Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI(Blue).
IF-P
IF analysis using anti- UBE2M/UBC12 antibody. detected in paraffin-embedded section of human breast cancer tissue. The tissue section were stained using the Dylight®550-conjugated Anti-rabbit IgG Secondary Antibody (red)and counterstained with DAPI (blue).
FC
Flow Cytometry analysis of Daudi cells using anti-UBC12/UBE2M antibody. Overlay histogram showing Daudi cells stained with anti-UBC12/UBE2M antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBC12/UBE2M Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
