Application
UBE2W Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow).Application
UBE2W Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow).Application
UBE2W Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE2W Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | UBE2W Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB,IHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UBC16 |
|---|---|
| Alternative Names | UBE2W UBC16 Ubiquitin-conjugating enzyme E2 W Ubiquitin carrier protein W Ubiquitin-conjugating enzyme 16 Ubiquitin-protein ligase W |
| Molecular Weight(MW) | 17331 Da |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination |
| Tissue Specificity | Widely expressed, with highest expression in brain, liver, pancreas and heart |
| Cellular Localization | Nucleus. Note=In the nucleus, colocalizes with FANCL |
| Entrez Gene | 55284 |
|---|---|
| Nucleotide Accession | NP_001001481.1 NP_060769.3 |
Application
UBE2W Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow).
Application
UBE2W Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow).
Application
UBE2W Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE2W Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
