Application
A. Western blot analysis on (A) HeLa and (B) A431 cell lysates using anti-UBF RabMAb (Cat. #AJ1806a).Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-UBF1 RabMAb (Cat. #AJ1806a).| Product Name | UBF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBTF, UBF, UBF1, Nucleolar transcription factor 1, Autoantigen NOR-90 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP2741Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:2000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UBF UBF1 |
|---|---|
| Alternative Names | UBTF UBF UBF1 Nucleolar transcription factor 1 Autoantigen NOR-90 |
| Molecular Weight(MW) | 89406 Da |
| Function | Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N-terminus of human UBF was used as an immunogen. |
| Cellular Localization | Nucleus, nucleolus (By similarity). |
| Entrez Gene | 7343 |
|---|
Application
A. Western blot analysis on (A) HeLa and (B) A431 cell lysates using anti-UBF RabMAb (Cat. #AJ1806a).
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-UBF1 RabMAb (Cat. #AJ1806a).| Application Notes | WB:1:2000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
