Application
Western blot analysis of anti-UBE4A Antibody (C-term) in NCI-H460 and CEM cell line lysates (35ug/lane). UBE4A (arrow) was detected using the purified Pab (1:60dilution).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | UBE4A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UBE4A; KIAA0126; Ubiquitin conjugation factor E4 A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~200 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0126 |
|---|---|
| Alternative Names | UBE4A KIAA0126 Ubiquitin conjugation factor E4 A |
| Molecular Weight(MW) | 122561 Da |
| Function | Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3 (By similarity) |
| Tissue Specificity | This UBE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1038~1067 amino acids from the C-terminal region of human UBE4A. |
Application
Western blot analysis of anti-UBE4A Antibody (C-term) in NCI-H460 and CEM cell line lysates (35ug/lane). UBE4A (arrow) was detected using the purified Pab (1:60dilution).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~200 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
