Application
The anti-UCHL5 Pab is used in Western blot to detect UCHL5 in mouse kidney tissue lysate.Application
HE staining of frozen human ovarian cancer tissue reacted with the primary antibody at a 1:250 dilution. Levels using the antibody on frozen tissue array (A )correlated well with the mRNA expression levels detected by Agilent expression microarray (B). This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles.| Product Name | UCH37 (UCHL5) Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UCHL5; UCH37; Ubiquitin carboxyl-terminal hydrolase isozyme L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UCH37 |
|---|---|
| Alternative Names | UCHL5 UCH37 Ubiquitin carboxyl-terminal hydrolase isozyme L5 Ubiquitin C-terminal hydrolase UCH37 Ubiquitin thioesterase L5 |
| Molecular Weight(MW) | 37607 Da |
| Function | Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 |
| Tissue Specificity | This UCH37 (UCHL5) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64~94 amino acids from the N-terminal region of human UCHL5. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus |
| Entrez Gene | 51377 |
|---|
Application
The anti-UCHL5 Pab is used in Western blot to detect UCHL5 in mouse kidney tissue lysate.
Application
HE staining of frozen human ovarian cancer tissue reacted with the primary antibody at a 1:250 dilution. Levels using the antibody on frozen tissue array (A )correlated well with the mRNA expression levels detected by Agilent expression microarray (B). This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
