Application
Western blot analysis of UCHL1 (arrow) using rabbit polyclonal UCHL1-V31 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UCHL1 gene (Lane 2) (Origene Technologies).Application
Western blot analysis of UCHL1-V31 in CEM(lane 1), Jurkat(lane 2), Y79(lane 3) cell line and mouse brain tissue(lane 4) lysates (35ug/lane). UCHL1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of UCHL1 Antibody (N-term)(Cat#AP2126a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
UCHL1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | UCHL1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UCHL1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Neuron cytoplasmic protein 9.5; PGP 9.5; Ubiquitin thioesterase L1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | UCHL1 Ubiquitin carboxyl-terminal hydrolase isozyme L1 Neuron cytoplasmic protein 9.5 PGP 9.5 Ubiquitin thioesterase L1 |
|---|---|
| Molecular Weight(MW) | 24824 Da |
| Function | Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes The homodimer may have ATP-independent ubiquitin ligase activity |
| Tissue Specificity | Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients |
| Cellular Localization | Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain |
| Entrez Gene | 7345 |
|---|
Application
Western blot analysis of UCHL1 (arrow) using rabbit polyclonal UCHL1-V31 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UCHL1 gene (Lane 2) (Origene Technologies).
Application
Western blot analysis of UCHL1-V31 in CEM(lane 1), Jurkat(lane 2), Y79(lane 3) cell line and mouse brain tissue(lane 4) lysates (35ug/lane). UCHL1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with UCHL1 (Park5) antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of UCHL1 Antibody (N-term)(Cat#AP2126a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
UCHL1 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
