Application
Western blot analysis of UCHL3 in HepG2 cell line lysates (35ug/lane). UCHL3 (arrow) was detected using the purified Pab.Application
Western blot analysis of his tag antibody in UCHL3 gene transfected 293 lysates (35ug/lane). His-tag UCHL3 protein (arrow) is detected.Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with UCHL3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with UCHL3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | UCHL3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UCHL3; Ubiquitin carboxyl-terminal hydrolase isozyme L3; Ubiquitin thioesterase L3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | UCHL3 Ubiquitin carboxyl-terminal hydrolase isozyme L3 Ubiquitin thioesterase L3 |
|---|---|
| Molecular Weight(MW) | 26183 Da |
| Function | Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders |
| Tissue Specificity | Highly expressed in heart, skeletal muscle, and testis |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 7347 |
|---|
Application
Western blot analysis of UCHL3 in HepG2 cell line lysates (35ug/lane). UCHL3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of his tag antibody in UCHL3 gene transfected 293 lysates (35ug/lane). His-tag UCHL3 protein (arrow) is detected.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with UCHL3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with UCHL3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
