Application
Western blot analysis of UCK2 (arrow) using rabbit polyclonal hUCK2-C241 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UCK2 gene.Application
UCK2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UCK2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
UCK2 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | UCK2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UCK2; UMPK; Uridine-cytidine kinase 2; Cytidine monophosphokinase 2; Testis-specific protein TSA903; Uridine monophosphokinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UMPK |
|---|---|
| Alternative Names | UCK2 UMPK Uridine-cytidine kinase 2 Cytidine monophosphokinase 2 Testis-specific protein TSA903 Uridine monophosphokinase 2 |
| Molecular Weight(MW) | 29299 Da |
| Function | Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4- thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)- benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5- methylcytidine, and N(4)-anisoylcytidine |
| Tissue Specificity | According to PubMed:8812458; testis-specific. According to PubMed:11306702, placenta-specific |
| Entrez Gene | 7371 |
|---|
Application
Western blot analysis of UCK2 (arrow) using rabbit polyclonal hUCK2-C241 . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UCK2 gene.
Application
UCK2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UCK2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
UCK2 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
