Application
Western blot analysis of UFD1L antibody (C-term) in K562 cell line lysates (35ug/lane). UFD1L (arrow) was detected using the purified Pab.Application
Western blot analysis of UFD1L(arrow) using rabbit polyclonal UFD1L Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UFD1L gene (Lane 2) (Origene Technologies).Application
Confocal immunofluorescent analysis of UFD1L Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | UFD1L Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UFD1L; Ubiquitin fusion degradation protein 1 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | UFD1L Ubiquitin fusion degradation protein 1 homolog |
|---|---|
| Molecular Weight(MW) | 34500 Da |
| Function | Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures |
| Tissue Specificity | Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney |
| Cellular Localization | Nucleus (By similarity). Cytoplasm, cytosol (By similarity) |
| Entrez Gene | 7353 |
|---|
Application
Western blot analysis of UFD1L antibody (C-term) in K562 cell line lysates (35ug/lane). UFD1L (arrow) was detected using the purified Pab.
Application
Western blot analysis of UFD1L(arrow) using rabbit polyclonal UFD1L Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UFD1L gene (Lane 2) (Origene Technologies).
Application
Confocal immunofluorescent analysis of UFD1L Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
