Application
UGT2B15 Antibody (Center) western blot analysis in NCI-H460(lane 1),HepG2(lane 2),ZR-75-1(lane 3) cell line lysates (35ug/lane).This demonstrates the UGT2B15 antibody detected the UGT2B15 protein (arrow).Application
UGT2B15 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UGT2B15 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Confocal immunofluorescent analysis of UGT2B15 Antibody (Center) (Cat#AP12375c) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
UGT2B15 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | UGT2B15 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UGT2B15; UGT2B8; UDP-glucuronosyltransferase 2B15; HLUG4; UDP-glucuronosyltransferase 2B8; UDPGTh-3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UGT2B8 |
|---|---|
| Alternative Names | UGT2B15 UGT2B8 UDP-glucuronosyltransferase 2B15 HLUG4 UDP-glucuronosyltransferase 2B8 UDPGTh-3 |
| Molecular Weight(MW) | 61036 Da |
| Function | UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme displays activity toward several classes of xenobiotic substrates, including simple phenolic compounds, 7-hydroxylated coumarins, flavonoids, anthraquinones, and certain drugs and their hydroxylated metabolites. It also catalyzes the glucuronidation of endogenous estrogens and androgens |
| Tissue Specificity | Expressed in many tissues. Present in liver, prostate and testis |
| Cellular Localization | Microsome membrane; Single-pass membrane protein (Potential). Endoplasmic reticulum membrane; Single-pass membrane protein (Potential) |
| Entrez Gene | 7366 |
|---|---|
| Nucleotide Accession | NP_001067.2 |
Application
UGT2B15 Antibody (Center) western blot analysis in NCI-H460(lane 1),HepG2(lane 2),ZR-75-1(lane 3) cell line lysates (35ug/lane).This demonstrates the UGT2B15 antibody detected the UGT2B15 protein (arrow).
Application
UGT2B15 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UGT2B15 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Confocal immunofluorescent analysis of UGT2B15 Antibody (Center) (Cat#AP12375c) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
UGT2B15 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
