Application
UMOD Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the UMOD antibody detected the UMOD protein (arrow).Application
UMOD Antibody (Center) (AP14256c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UMOD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | UMOD Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UMOD; Uromodulin; Tamm-Horsfall urinary glycoprotein; Uromodulin, secreted form |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | UMOD Uromodulin Tamm-Horsfall urinary glycoprotein Uromodulin secreted form |
|---|---|
| Molecular Weight(MW) | 69761 Da |
| Function | Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure providing the water barrier permeability. May serve as a receptor for binding and endocytosis for cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelial |
| Tissue Specificity | Synthesized exclusively in the kidney. Expressed exclusively by epithelial cells of the thick ascending limb of Henle's loop (TALH) and of distal convoluted tubule lumen Most abundant protein in normal urine |
| Cellular Localization | Apical cell membrane; Lipid-anchor, GPI- anchor. Basolateral cell membrane; Lipid-anchor, GPI-anchor. Cell projection, cilium membrane. Note=Only a small fraction is sort to the basolateral pole of tubular epithelial cells compared to apical localization. Secreted into urine after cleavage Colocalized with NPHP1 and KIF3A |
| Entrez Gene | 7369 |
|---|---|
| Nucleotide Accession | NP_003352.2 NP_001008390.1 |
Application
UMOD Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the UMOD antibody detected the UMOD protein (arrow).
Application
UMOD Antibody (Center) (AP14256c)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UMOD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
