Application
Western blot analysis of anti-ULK3 Antibody (N-term) in mouse heart tissue lysates (35ug/lane).ULK3(arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with *ULK3 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | ULK3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ULK3; Serine/threonine-protein kinase ULK3; Unc-51-like kinase 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ULK3 Serine/threonine-protein kinase ULK3 Unc-51-like kinase 3 |
|---|---|
| Molecular Weight(MW) | 53444 Da |
| Function | Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy |
| Tissue Specificity | Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord |
| Cellular Localization | Cytoplasm. Note=Localizes to pre- autophagosomal structure during cellular senescence |
| Entrez Gene | 25989 |
|---|
Application
Western blot analysis of anti-ULK3 Antibody (N-term) in mouse heart tissue lysates (35ug/lane).ULK3(arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with *ULK3 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
