Application
UPF2 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the UPF2 antibody detected the UPF2 protein (arrow).Application
UPF2 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | UPF2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UPF2; KIAA1408; RENT2; Regulator of nonsense transcripts 2; Nonsense mRNA reducing factor 2; Up-frameshift suppressor 2 homolog |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1408 RENT2 |
|---|---|
| Alternative Names | UPF2 KIAA1408 RENT2 Regulator of nonsense transcripts 2 Nonsense mRNA reducing factor 2 Up-frameshift suppressor 2 homolog |
| Molecular Weight(MW) | 147810 Da |
| Function | Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Cytoplasm, perinuclear region. |
| Entrez Gene | 26019 |
|---|---|
| Nucleotide Accession | NP_056357.1 |
Application
UPF2 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the UPF2 antibody detected the UPF2 protein (arrow).
Application
UPF2 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
