Application
UPF3A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UPF3A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
UPF3A Antibody (Center) western blot analysis in HL-60,Jurkat,HepG2 cell line lysates (35ug/lane).This demonstrates the UPF3A antibody detected the UPF3A protein (arrow).| Product Name | UPF3A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UPF3A; RENT3A; UPF3; Regulator of nonsense transcripts 3A; Nonsense mRNA reducing factor 3A; Up-frameshift suppressor 3 homolog A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RENT3A UPF3 |
|---|---|
| Alternative Names | UPF3A RENT3A UPF3 Regulator of nonsense transcripts 3A Nonsense mRNA reducing factor 3A Up-frameshift suppressor 3 homolog A |
| Molecular Weight(MW) | 54696 Da |
| Function | Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation |
| Tissue Specificity | Isoform 1 is strongly expressed in testis, uterus, muscle, fetal brain and spinal cord. Isoform 2 is strongly expressed in fetal brain and spinal cord |
| Cellular Localization | Nucleus. Cytoplasm. Note=Shuttling between the nucleus and the cytoplasm |
| Entrez Gene | 65110 |
|---|---|
| Nucleotide Accession | NP_542418.1 |
Application
UPF3A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UPF3A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
UPF3A Antibody (Center) western blot analysis in HL-60,Jurkat,HepG2 cell line lysates (35ug/lane).This demonstrates the UPF3A antibody detected the UPF3A protein (arrow).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
