Application
Western blot analysis of UPF3B Antibody (Center) in Hela cell line lysates (35ug/lane). UPF3B (arrow) was detected using the purified Pab.| Product Name | UPF3B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UPF3B; RENT3B; UPF3X; Regulator of nonsense transcripts 3B; Nonsense mRNA reducing factor 3B; Up-frameshift suppressor 3 homolog B; Up-frameshift suppressor 3 homolog on chromosome X |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RENT3B UPF3X |
|---|---|
| Alternative Names | UPF3B RENT3B UPF3X Regulator of nonsense transcripts 3B Nonsense mRNA reducing factor 3B Up-frameshift suppressor 3 homolog B Up-frameshift suppressor 3 homolog on chromosome X |
| Molecular Weight(MW) | 57762 Da |
| Function | Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2- UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is indepenedent of association with UPF2 and components of the EJC core |
| Tissue Specificity | Expressed in testis, uterus, prostate, heart, muscle, brain, spinal cord and placenta |
| Cellular Localization | Nucleus. Cytoplasm. Note=Shuttling between the nucleus and the cytoplasm |
| Entrez Gene | 65109 |
|---|
Application
Western blot analysis of UPF3B Antibody (Center) in Hela cell line lysates (35ug/lane). UPF3B (arrow) was detected using the purified Pab.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
