Application
UQCRFS1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UQCRFS1 antibody detected the UQCRFS1 protein (arrow).Application
UQCRFS1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UQCRFS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
UQCRFS1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of UQCRFS1 Antibody (C-term)(Cat#AP11659b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | UQCRFS1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UQCRFS1; Cytochrome b-c1 complex subunit Rieske, mitochondrial; Complex III subunit 5; Cytochrome b-c1 complex subunit 5; Rieske iron-sulfur protein; Ubiquinol-cytochrome c reductase iron-sulfur subunit; Cytochrome b-c1 complex subunit 11; Complex III sub |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | UQCRFS1 Cytochrome b-c1 complex subunit Rieske mitochondrial Complex III subunit 5 Cytochrome b-c1 complex subunit 5 Rieske iron-sulfur protein Ubiquinol-cytochrome c reductase iron-sulfur subunit Cytochrome b-c1 complex subunit 11 Complex III sub |
|---|---|
| Molecular Weight(MW) | 29668 Da |
| Function | Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis |
| Tissue Specificity | This UQCRFS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the C-terminal region of human UQCRFS1. |
| Cellular Localization | Mitochondrion inner membrane. |
| Entrez Gene | 7386 |
|---|---|
| Nucleotide Accession | NP_005994.2 |
Application
UQCRFS1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UQCRFS1 antibody detected the UQCRFS1 protein (arrow).
Application
UQCRFS1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UQCRFS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
UQCRFS1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of UQCRFS1 Antibody (C-term)(Cat#AP11659b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
