Application
USF1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USF1 antibody detected the USF1 protein (arrow).Application
USF1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Fluorescent confocal image of SK-BR-3 cell stained with USF1 Antibody (Center)(Cat#AP11078c). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with USF1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).USF1 immunoreactivity is localized to nucleus significantly.Application
USF1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | USF1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | USF1; BHLHB11; USF; Upstream stimulatory factor 1; Class B basic helix-loop-helix protein 11; Major late transcription factor 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIFIHC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHB11 USF |
|---|---|
| Alternative Names | USF1 BHLHB11 USF Upstream stimulatory factor 1 Class B basic helix-loop-helix protein 11 Major late transcription factor 1 |
| Molecular Weight(MW) | 33538 Da |
| Function | Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters |
| Tissue Specificity | This USF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-201 amino acids from the Central region of human USF1. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 7391 |
|---|---|
| Nucleotide Accession | NP_009053.1 |
Application
USF1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the USF1 antibody detected the USF1 protein (arrow).
Application
USF1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Fluorescent confocal image of SK-BR-3 cell stained with USF1 Antibody (Center)(Cat#AP11078c). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with USF1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).USF1 immunoreactivity is localized to nucleus significantly.
Application
USF1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of USF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
